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Clinical and genomic features associated with increasing <t>ATM/CZA</t> and AZA MICs. The X -axis is sorted first by increasing ATM/CZA <t>MIC</t> and then by increasing ATM and CZA MIC, respectively. Y -axis from top to bottom indicates: Rows 1–5: presence/absence of chromosomal mutations predicted to impact β-lactam resistance; Rows 6–11: presence/absence and copy number variation (CNV) of exogenous B-lactamase content; Rows 12–14: Clinical metadata. Vertical dotted red line indicates the cutoff between ATM/CZA-S (left) and ATM/CZA-NS (right). Metadata from the heatmap is labeled accordingly in the legend present to the right of the figure. Note for ftsI mutations, dark purple indicates predicted YRIN mutation, whereas light purple indicates predicted YRIK mutation. The striped ftsI rectangle indicates a point mutation (p.A498T) associated with cephalosporin resistance as annotated in AMRFinderPlus .
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Clinical and genomic features associated with increasing ATM/CZA and AZA MICs. The X -axis is sorted first by increasing ATM/CZA MIC and then by increasing ATM and CZA MIC, respectively. Y -axis from top to bottom indicates: Rows 1–5: presence/absence of chromosomal mutations predicted to impact β-lactam resistance; Rows 6–11: presence/absence and copy number variation (CNV) of exogenous B-lactamase content; Rows 12–14: Clinical metadata. Vertical dotted red line indicates the cutoff between ATM/CZA-S (left) and ATM/CZA-NS (right). Metadata from the heatmap is labeled accordingly in the legend present to the right of the figure. Note for ftsI mutations, dark purple indicates predicted YRIN mutation, whereas light purple indicates predicted YRIK mutation. The striped ftsI rectangle indicates a point mutation (p.A498T) associated with cephalosporin resistance as annotated in AMRFinderPlus .

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Clinical and genomic determinants associated with emergent ceftazidime–avibactam plus aztreonam non-susceptibility in ceftazidime-avibactam resistant Escherichia coli

doi: 10.1128/aac.01860-25

Figure Lengend Snippet: Clinical and genomic features associated with increasing ATM/CZA and AZA MICs. The X -axis is sorted first by increasing ATM/CZA MIC and then by increasing ATM and CZA MIC, respectively. Y -axis from top to bottom indicates: Rows 1–5: presence/absence of chromosomal mutations predicted to impact β-lactam resistance; Rows 6–11: presence/absence and copy number variation (CNV) of exogenous B-lactamase content; Rows 12–14: Clinical metadata. Vertical dotted red line indicates the cutoff between ATM/CZA-S (left) and ATM/CZA-NS (right). Metadata from the heatmap is labeled accordingly in the legend present to the right of the figure. Note for ftsI mutations, dark purple indicates predicted YRIN mutation, whereas light purple indicates predicted YRIK mutation. The striped ftsI rectangle indicates a point mutation (p.A498T) associated with cephalosporin resistance as annotated in AMRFinderPlus .

Article Snippet: Additionally, for this study, gradient cross-strip testing was performed for ATM/CZA using CZA MIC test strips (Liofilchem, Italy) and ATM ETEST strips (bioMérieux, Marcy-l'Étoile, France) using our previously established and validated method ( ).

Techniques: Labeling, Mutagenesis